Journal: The Plant Cell

Article Title: The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera ^[W]

doi: 10.1105/tpc.113.117127

Figure Lengend Snippet: Expression of MYB14, MYB15, STSs, and Stilbene Accumulation during Grape Berry Development of V. vinifera cv Pinot Noir. (A) Columns represent MYB and STS transcript profiles in flower/skins. (B) Stilbene accumulation in flower/skins. (C) MYB and STS transcript profiles in seeds. (D) Stilbene accumulation in seeds. Data points are given as weeks from the onset of ripening (véraison; labeled with an arrow), with expression values as means of three replicate PCRs (n = 6) and error bars indicating se. Note that from 6 weeks before véraison (-6), berry skin has been separated from seeds. Flowering occurred 8 weeks before véraison. Accumulation of stilbenes was measured as methanol extracts of developing grape tissues using reverse-phase HPLC analysis. (B) and (D) show relative stilbene contents of trans-piceid in developing berries and in seeds as the mean of three different experiments, with error bars indicating se. Note that each time point during developmental series is a pool of >100 berries collected from ∼20 plants growing as described in Methods. FW, fresh weight.

Article Snippet: HPLC analysis of stilbene compounds was performed on a reverse-phase HPLC device (Kontron Instruments; 322 pump system/360 autosampler/335 HPLC detector) with a Symmetry C18 column (3.5 μm, 4.6 mm × 150 mm [WAT200632]; Waters) protected by a guard column ( Czemmel et al., 2009 ).

Techniques: Expressing, Labeling